VALERIE
Visualising Splicing at Single-Cell Resolution
Description
Alternative splicing produces a variety of different protein products from a given gene. 'VALERIE' enables visualisation of alternative splicing events from high-throughput single-cell RNA-sequencing experiments. 'VALERIE' computes percent spliced-in (PSI) values for user-specified genomic coordinates corresponding to alternative splicing events. PSI is the proportion of sequencing reads supporting the included exon/intron as defined by Shiozawa (2018) <doi:10.1038/s41467-018-06063-x>. PSI are inferred from sequencing reads data based on specialised infrastructures for representing and computing annotated genomic ranges by Lawrence (2013) <doi:10.1371/journal.pcbi.1003118>. Computed PSI for each single cell are subsequently presented in the form of a heatmap implemented using the 'pheatmap' package by Kolde (2010) <https://CRAN.R-project.org/package=pheatmap>. Board overview of the mean PSI difference and associated p-values across different user-defined groups of single cells are presented in the form of a line graph using the 'ggplot2' package by Wickham (2007) <https://CRAN.R-project.org/package=ggplot2>.
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| r-devel-windows-x86_64 | OK |
| r-oldrel-macos-arm64 | NOTE |
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| r-release-windows-x86_64 | OK |
Check details (15 non-OK)
CRAN incoming feasibility
Maintainer: ‘Sean Wen <sean.wenwx@gmail.com>’
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Authors@R: person(given = "Sean",
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email = "sean.wenwx@gmail.com")
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package dependencies
Packages required but not available: 'GenomicAlignments', 'Rsamtools' See section ‘The DESCRIPTION file’ in the ‘Writing R Extensions’ manual.
CRAN incoming feasibility
Maintainer: ‘Sean Wen <sean.wenwx@gmail.com>’
No Authors@R field in DESCRIPTION.
Please add one, modifying
Authors@R: person(given = "Sean",
family = "Wen",
role = c("aut", "cre"),
email = "sean.wenwx@gmail.com")
as necessary.
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installed package size
installed size is 9.4Mb
sub-directories of 1Mb or more:
extdata 8.7Mb
installed package size
installed size is 9.4Mb
sub-directories of 1Mb or more:
extdata 8.7Mb
installed package size
installed size is 9.5Mb
sub-directories of 1Mb or more:
extdata 8.8Mb
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ERROR 9 OK · 4 NOTE · 0 WARNING · 1 ERROR · 0 FAILURE Mar 9, 2026
CRAN incoming feasibility
Maintainer: ‘Sean Wen <sean.wenwx@gmail.com>’
No Authors@R field in DESCRIPTION.
Please add one, modifying
Authors@R: person(given = "Sean",
family = "Wen",
role = c("aut", "cre"),
email = "sean.wenwx@gmail.com")
as necessary.
CRAN incoming feasibility
Maintainer: ‘Sean Wen <sean.wenwx@gmail.com>’
No Authors@R field in DESCRIPTION.
Please add one, modifying
Authors@R: person(given = "Sean",
family = "Wen",
role = c("aut", "cre"),
email = "sean.wenwx@gmail.com")
as necessary.
installed package size
installed size is 9.4Mb
sub-directories of 1Mb or more:
extdata 8.7Mb
installed package size
installed size is 9.4Mb
sub-directories of 1Mb or more:
extdata 8.7Mb
installed package size
installed size is 9.5Mb
sub-directories of 1Mb or more:
extdata 8.8Mb