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HetSeq

Identifying Modulators of Cellular Responses Leveraging Intercellular Heterogeneity

v0.1.0 · Feb 3, 2025 · Apache License (>= 2)

Description

Cellular responses to perturbations are highly heterogeneous and depend largely on the initial state of cells. Connecting post-perturbation cells via cellular trajectories to untreated cells (e.g. by leveraging metabolic labeling information) enables exploitation of intercellular heterogeneity as a combined knock-down and overexpression screen to identify pathway modulators, termed Heterogeneity-seq (see 'Berg et al' <doi:10.1101/2024.10.28.620481>). This package contains functions to generate cellular trajectories based on scSLAM-seq (single-cell, thiol-(SH)-linked alkylation of RNA for metabolic labelling sequencing) time courses, functions to identify pathway modulators and to visualize the results.

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OK 14 OK · 0 NOTE · 0 WARNING · 0 ERROR · 0 FAILURE Mar 9, 2026

Dependency Network

Dependencies Reverse dependencies cowplot doParallel foreach DoubleML e1071 igraph ggplot2 ggrastr ggrepel grandR lpSolve mlr3 pROC reshape2 scales +1 more dependencies HetSeq

Version History

new 0.1.0 Mar 9, 2026