AmpliconDuo
1.1.1Statistical Analysis of Amplicon Data of the Same Sample to Identify Artefacts
Overview
Increasingly powerful techniques for high-throughput sequencing open the possibility to comprehensively characterize microbial communities, including rare species. However, a still unresolved issue are the substantial error rates in the experimental process generating these sequences. To overcome these limitations we propose an approach, where each sample is split and the same amplification and sequencing protocol is applied to both halves. This procedure should allow to detect likely PCR and sequencing artifacts, and true rare species by comparison of the results of both parts. The AmpliconDuo package, whereas amplicon duo from here on refers to the two amplicon data sets of a split sample, is intended to help interpret the obtained read frequency distribution across split samples, and to filter the false positive reads.
Install
Health
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- NOTE2026-03-100 OK · 14 NOTE · 0 WARNING · 0 ERROR · 0 FAILURE
Documentation
- Examples that run
- 100%
- Documented parameters
- 97%
- Return-value docs
- 63%
- References docs
- 62%
Downloads
Dependencies
Nothing depends on this yet.
Code & Tests
Datasets
People & History
3 releases. Pick two to compare their code metrics. R releases are shown for context.
- RR 4.6.0 released · 2026-04-24
- RR 4.5.0 released · 2025-04-11
- RR 4.4.0 released · 2024-04-24
- RR 4.3.0 released · 2023-04-21
- RR 4.2.0 released · 2022-04-22
- RR 4.1.0 released · 2021-05-18
- 1.1.1Latest
- unarchivedReturned to CRAN2020-05-26
- RR 4.0.0 released · 2020-04-24
- archivedRemoved from CRAN2020-01-14check errors were not corrected in time Detritus with variable names
- RR 3.6.0 released · 2019-04-26
- RR 3.5.0 released · 2018-04-23
- RR 3.4.0 released · 2017-04-21
- RR 3.3.0 released · 2016-05-03
- 1.12016-01-19 · diff ↗
- RR 3.2.0 released · 2015-04-16
Show 3 earlier events
- RR 3.1.0 released · 2014-04-10
- 1.02014-03-20
- RR 3.0.0 released · 2013-04-03
Package metadata
- First published
- 2014-03-20
- Total releases
- 3 / 12 yrs
- License
- GPL (>= 3) OSI
- Minimum R
- ≥ 2.10
- Bundled data
- 447 KB / 4 files
- Download size
- 483 KB
- Installed size
- not tracked yet
- With dependencies
- not tracked yet