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FracFixR

Compositional Statistical Framework for RNA Fractionation Analysis

v1.0.0 · Oct 21, 2025 · CC BY 4.0

Description

A compositional statistical framework for absolute proportion estimation between fractions in RNA sequencing data. 'FracFixR' addresses the fundamental challenge in fractionated RNA-seq experiments where library preparation and sequencing depth obscure the original proportions of RNA fractions. It reconstructs original fraction proportions using non-negative linear regression, estimates the "lost" unrecoverable fraction, corrects individual transcript frequencies, and performs differential proportion testing between conditions. Supports any RNA fractionation protocol including polysome profiling, sub-cellular localization, and RNA-protein complex isolation.

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CRAN Check Status

3 FAILURE
11 OK
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r-devel-linux-x86_64-debian-clang OK
r-devel-linux-x86_64-debian-gcc OK
r-devel-linux-x86_64-fedora-clang OK
r-devel-linux-x86_64-fedora-gcc OK
r-devel-macos-arm64 OK
r-devel-windows-x86_64 FAILURE
r-oldrel-macos-arm64 OK
r-oldrel-macos-x86_64 OK
r-oldrel-windows-x86_64 FAILURE
r-patched-linux-x86_64 OK
r-release-linux-x86_64 OK
r-release-macos-arm64 OK
r-release-macos-x86_64 OK
r-release-windows-x86_64 FAILURE
Check details (14 non-OK)
OK r-devel-linux-x86_64-debian-clang

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OK r-devel-linux-x86_64-debian-gcc

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OK r-devel-linux-x86_64-fedora-clang

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OK r-devel-linux-x86_64-fedora-gcc

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OK r-devel-macos-arm64

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FAILURE r-devel-windows-x86_64

re-building of vignette outputs


            
OK r-oldrel-macos-arm64

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OK r-oldrel-macos-x86_64

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FAILURE r-oldrel-windows-x86_64

re-building of vignette outputs


            
OK r-patched-linux-x86_64

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OK r-release-linux-x86_64

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OK r-release-macos-arm64

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OK r-release-macos-x86_64

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FAILURE r-release-windows-x86_64

re-building of vignette outputs


            

Check History

OK 11 OK · 0 NOTE · 0 WARNING · 0 ERROR · 3 FAILURE Mar 9, 2026
FAILURE r-devel-windows-x86_64

re-building of vignette outputs

FAILURE r-release-windows-x86_64

re-building of vignette outputs

FAILURE r-oldrel-windows-x86_64

re-building of vignette outputs

Dependency Network

Dependencies Reverse dependencies future.apply nnls ggplot2 dplyr RColorBrewer tidyr matrixStats aod future FracFixR

Version History

new 1.0.0 Mar 10, 2026